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WPI Duo 773 Electrometer

Dual channel intracellular amplifier for intracellular dual or differential studies

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  • 2-channel intracellular amplifier
  • For intracellular dual or differential studies
  • Built-in active filtering
  • Comes complete with 2 probe headstages, normally one high impedance and one low impedance
  • Optional configuration: Two low impedance balanced probes

Headstage
For intracellular dual or diferential studies, the Duo773 has separate negative capacity controls and built-in active filtering that allows the precise balancing of time constants for artifact-free differential measurement. Comes complete with two probe headstages, 1015 Ohms and 1011 Ohms probes to monitor signals from ion-specific micro-electrodes as well as KCl-filled electrodes. Two-gold plated, epoxy sealed miniature active probes can be positioned directly to the measurement site. Microelectrode holders containing an Ag/AgCl electrochemical half-cells plug directly into the probes. Stray capacitance can be reduced by placing the included driven guard shield over the microelectrode holder at the end of the probe.

Capacity Compensation
Channel A can compensate up to 10pF of electrode shunt capacity and Channel B can compensate up to 50pF.

Tickler Circuit
Assists in cell penetration. The frequency and amplitude of the oscillations may be varied for differences in membrane thickness or cell size. The duration of tickle can be controlled either by using the momentary switch, a foot switch, or by applying a signal to the remote tickler input.

Active Filters
Low pass settings on a -40dB/decade active filter vary the cutoff from 1 to 30 kHz. Either probe or bridge outputs may be selected for filtering.

Current Injection
Channel B can eject current through the microelectrode by applying a command signal to the stimulus input connector; the resulting output from the probe will then be a constant current replica of the input signal. Two ranges of current delivery are provided: 50nA and 500nA or by an external source. This source can be useful for delivering hyperpolarising currents to stabilise the cell membrane potential and as a holding current for microiontophoresis.