Designed to simplify intracellular injection and a variety of other microinjection tasks, the PicoPump from WPI use carefully regulated air pressures for securing cells and injecting them with fluid. Injected volumes range from picolitres to nanolitres. Separate ports supply positive and negative pressure - positive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip.
- Regulated Hold, Ejection and Vacuum Pressure
- Carefully regulated air pressues for securing cells and injecting them with fluid
- Pressure input 0-150psi
- Pressure output 0.3-90psi
A second pressure port maintains a low positive "holding" pressure to the injecting pipette between injection pulses, to prevent fluid uptake through capillary action or diffusion. Timing, ejection pressure, holding pressure, and suction are adjusted independently by control knobs and indicator gauges on the front panel. Injection pressure is controlled by a 20-turn regulator on the front panel. A built-in timing circuit allows precise control of the amount of time that the injection pressure is applied to the output port. Time intervals can range from 10 seconds down to 10 ms or less, depending on the eject pressure setting. The injection pressure interval can be triggered manually on the front panel, by footswitch, or by computer controlled TTL pulse. A 5 volt monitor output provides a logic-level pulse for your computer or other monitoring device.