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Nikon Ti-LAPP Modular Illumination System

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Nikon Ti-LAPP Modular Illumination System

Nikon Ti-LAPP Modular Illumination System

Modular illumination system with ultimate flexibility and expandability


The Nikon Ti-LAPP system provides modular illuminators for total internal reflection fluorescence (TIRF), photoactivation/conversion, photobleaching and epi-fluorescence. Each module can be flexibly combined to build microscope systems that are optimised for individual research needs. For example, multiple TIRF modules can be incorporated into a single microscope for anisotropy experiments and fast, multi-angle TIRF imaging. Combined with the Ti's stratum structure, up to five illumination modules can be incorporated into a single microscope (e.g. two TIRFs, a FRAP, a DMD and an Epi-FL module can all be integrated into one Ti).

DMD Module - Achieves Simultaneous Multipoint Photoactivation
The new DMD module enables photoactivation and photoconversion of a user-specified pattern and position(s), whereas the conventional FRAP unit only enables photoactivation of a single, manually-positioned spot. The DMD illumination shape, size, position and number can be freely customised using the NIS-Elements software. This capability allows researchers to optically mark a subset of cells or protein populations within a single cell or multiple cells to track their behavior.

H-TIRF Module - Fully Automated TIRF Adjustment and Observation is Now Possible
The ideal incident angle and focus of the laser for TIRF observation vary depending on specimen and observation conditions. Adjusting the incident angle and focus for achieving TIRF requires skill and experience. The new H-TIRF module automatically adjusts the focus and incident angle of the laser for TIRF observation by monitoring the reflection beam. This automatic laser focus adjustment and incident angle adjustment is carried out by the auto-alignment function in NIS-Elements software.

N-STORM Module - Achieves a Resolution 10 times Greater than Conventional Optical Microscopy

Equipped with illumination field (1x, 2x, 4x) motorized switching, as well as an auto-alignment function, this module enables N-STORM super-resolution imaging with the Ti2-LAPP system. This delivers an incredible image resolution of approximately 20 nm, which is 10 times or greater than the limit in conventional optical microscopy. Utilizing STochastic Optical Resolution Microscopy (STORM), it is possible to gain insight into protein-protein interactions at a molecular level.

FRAP Module - For Analysis of Intracellular-protein Dynamics
With this FRAP module, photobleaching and photoactivation/conversion experiments coupled with the use of high-frame-rate, high-sensitivity cameras for detection are possible. This module can spot-illuminate a target position in the cell, providing a cost-effective means for the study of intracellular protein dynamics without the use of a point-scanning confocal microscope.

TIRF Module - For Observation of Cell Membrane Dynamics and Single Molecules
The newly designed manual TIRF module includes a gradation ND filter (similar to the H-TIRF module), enabling even TIRF illumination across the field of view. Using high-sensitivity cameras, one can image single molecules and dynamics of proteins in and near the cell membrane using this TIRF illuminator.

XY Galvano Scanning Unit - For Simultaneous Photostimulation and Confocal Imaging

The XY galvano scanning unit is a photostimulation module that allows the user to stimulate the desired area of a sample through point scanning by means of laser. When mounted on the Ti2-E together with the A1 HD25/A1R HD25 confocal microscope, it allows acquisition of confocal images of live-cell dynamics while simultaneously carrying out photostimulation within a sample.

For further information please contact us or download the datasheet.

Read more on Nikon's website                                              Microscope Objectives brochure

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