Webinar : AFM Application in Single Molecule Study
Thursday 4 July 2019
Auckland (NZST) : 5:30pm
Brisbane, Sydney, Canberra, Melbourne, Hobart (AEST) : 3:30pm
Adelaide (ACST) : 3pm
Perth (AWDT) : 1:30pm
Cells can sense, adapt to, and even remodel their extracellular microenvironment. The interplay between the cell and its environment involves multiplex signaling networks, in which many molecules dully implement their own functions. Using traditional biochemical techniques, the molecular function can only be investigated based on the averaged activities of a large amount of molecules; while single molecule techniques can provide more detailed information by resolving the structure and interaction of each individual molecule. For example, AFM can reveal how many different conformations of Aβ amyloid fibrils existing at a specific condition, and how each conformation interacts with the monomeric protein. The monomer-monomer or monomer-fibril interaction variation can be monitored in-situ while the environmental conditions (e.g., pH and ionic strength) are changed.
On the other hand, force has been utilised by nature to drive protein conformational changes, therefore modulate its stability and functionality. Such mechanical mechanisms are widely involved in physiological events at cellular level. To understand these mechanisms at the molecular level, it is necessary to manipulate single bio-molecules and resolve sub-pN forces. Thanks to recent improvements in spatial, temporal, and force resolutions, AFM and optical tweezers are capable to address the above requests. Now the single molecule stretching and rupture events can be directly resolved with sub-nm distance and pN force resolutions. The mechanical stability of processive motors (e.g., kinesin and myosin) have been widely studied with optical tweezers. Subtle forces have been applied on ion-binding proteins and antigen-antibody complexes to understand the mechanical effect on the binding affinity.
In this webinar, Bruker will share the recent technological developments in this field and some examples in molecular applications of AFM and optical tweezers.Read More
Introducing the Nikon SoRa High Speed SR SD Confocal Microscope
Nikon has released their new SoRa high speed super-resoution microscope.
Based on a Yokogawa CSU-W1 spinning disc confocal platform the SoRa achieves 120nm lateral resolution through optical pixel realignment.
When combined with Nikon's award winning Eclipse Ti2-E inverted microscope and NIS-Elements software the system provides a high-speed, super-resolution microscope with powerful image automation and acquisition via Nikon JOB's and HCA functionality for super-resolution live-cell imaging.
The system includes two spinning discs which can be exchanged easily via software control - a super reolution disc which also includes the capability of optical sectioning, and a standard confocal imaging disc.Read More
WPI End of Financial Year Sale Starts Today !
10% discount is available from now until the end of June on WPI's entire catalogue product range. Contact Tim Watts (08) 8150 5289 for a quotation or to place an order today.
Sale ends Friday 28 June 2019
2019 Laboratory Equipment Catalogue 2019 Surgical Instruments Catalogue
2018 Sterilisation Catalogue 2017 Animal Physiology CatalogueRead More
Webinar : Unravelling Dynamic Processes with the New JPK NanoWizard ULTRA Speed 2 BioAFM
Wednesday 4 June 2019
New Zealand (NZST) : 7pm
Brisbane/Sydney/Canberra/Melbourne/Hobart (AEST) : 5pm
Perth (AWST) : 5pm
AFM has become an indispensable tool for the structural and mechanical analysis of samples ranging from single molecules to complex biological systems in life science and biophysics labs worldwide. It provides outstanding lateral and spatial resolution and does not require specific sample preparation or modification. It is currently being used in simultaneous, correlative studies with advanced optical and super-resolution techniques.
The JPK NanoWizard ULTRA Speed 2 meets the rising demand for novel developments and applications which enable the study of dynamic processes and kinetics in macromolecular systems with high temporal resolution. With line rates of 600 Hz and video rate imaging of up to 10 frames per second, it provides outstanding performance.
We will focus on the following applications:
- Accessing challenging biological samples, e.g. highly corrugated specimen
- The dissolution of calcite crystal plane terraces
- Thermodynamic de-/rehybridization of double-helical DNA
- Melting/crystallization of polyester polycaprolactone (PCL) films, and
- Fibroblast cells measured in liquid
Speaker : Dr Dimitar Stamov, Senior Applications Scientist at JPK BioAFM, Bruker Nano GmbH, has extensive experience in BioAFM technology and correlative techniques for the Life SciencesRead More
WPI 6-axis Motorised Stereotaxic Frame
New from WPI the MTM-6 is a 6-axis motorised stereotaxic frame which includes both manipulator arms and dual controllers, allowing you to mount, for example, a stereotaxic drill and probe simultaneously.
The motorised stereotaxic frame with a digital display outperforms manual versions, and greatly reduces human error when performing routine stereotaxic surgery. The motorised axes of the MTM-6 provide precise, controlled, 3-dimensional placement of any probe or accessory within the working space of the stereotaxic frame.Read More