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New Upright Microscope

Nikon Corporation is pleased to announce the release of the Eclipse Ci-L plus biological microscope.

 

Long term microscope users know all too well the issues of back and neck strain along with eye fatigue associated with operating and observing specimens under a microscope every day. To reduce these common fatigue problems, Nikon has designed the Eclipse Ci-L plus biological microscope with the intent of eliminating unnecessary operator movements while maintaining the high optical performance of the existing model, the Eclipse Ci-L. The Eclipse Ci-L plus enables users to concentrate on their observations and helps reduce fatigue associated with repetitive microscope operation.

 

Features:

  • LIM (Light Intensity Management) helps reduce eye strain by preventing sudden light intensity changes after changing magnifications
  • Ergonomic design contributes to reducing physical strain
  • Front panel display enables confirmation of magnification and illumination status at a glance
  • Scale bar adjustment linked to nosepiece rotation for more efficient observation
  • Automatic shut-off with ECO mode

 

For further information please contact us or read more.

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Supercharged Calcium Imaging

CoolLED are excited to introduce their new LED microscope illuminator for lightning-fast calcium imaging, pH monitoring, rtiometric fluorescence, FRET and everyday fluorescence micrsocopy, the pE-800fura

 

Fluorescence freedom : 8 powerful LED's span 340-635nm to cover all popular calcium and pH indicators, fluorophores and opsins from Fura-2 to Cy5.

Lightning-fast microscopy : Capture dynamic events at the highest temporal resolution and enhance the accuracy of ratiometric measurements with <7µs TTl triggering

Affordable automation: Inline excitation filters and our unique Sequence Runner for triggering illumination sequences with just a single TTL-out of a camera transforms a manual microscope into a powerful automated imaging system.
Low phototoxicity: Comprehensive controls such as fine irradiance modulation and individual channel switching protect samples from phototoxicity and photobleaching, also pushing the boundaries of time-lapse imaging.

Simply better control: In addition to electronic, analogue and GUI control via The LightBridge, full support is available in several imaging software platforms, including NIS Elements and µManager. Integration in cellSens is achieved using the pE-6501-8-FR USB controlled TTL trigger box.

 

Fur further information please read more, contact us or watch the video tutorials.

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Fast Line-scanning Confocal Microscope

Welcome to the family NL5 !

  • Fast scanning speed: 25fps
  • Perfect for deep 3D confocal imaging
  • Live-cell friendly
  • Easy to use & compact
  • Add-on to existing microscope (inverted and upright), left or right port

For further infromation please contact us, read more or book an online demo.

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AX AXR Confocal Microscope

Nikon Corporation (Nikon) is pleased to announce the release of the next generation confocal microscope series, AX and AX R. This new confocal series features a completely re-designed scan head with 8K x 8K resolution*1, ultra-high speed resonant scanning, and world’s largest*2 25 mm field of view*3. Combined with a new user-friendly interface and advanced AI-based tools, the AX/AX R confocal series is designed to enable users to acquire data faster, with an unprecedented level of detail and ease.

 

1. Capture more data than ever before with 8K x 8K pixel density and a 25 mm field of view

The AX and AX R enables 8192 x 8192 pixel, high-resolution images to be obtained with the world’s largest field of view (25 mm). Now, ultrafine details can be easily captured even for large specimens. This feature is available for both inverted*4 and upright*5 microscope configurations, thereby supporting a wide range of research applications and fields.

 

In addition, with the AX R’s high-speed resonant scanner users can acquire up to 720 frames per second at 2048 x 16 pixels. High-speed resonant scanning not only enables dynamic events to be easily captured but also reduces the amount of time required to image large, fixed specimens.

Time-lapse Z series maximum intensity projection images of a developing Drosophila embryo expressing PLC-PH::GFP (PIP2) acquired every 10 minutes for 12 hours with 2K x 1K pixels (with 25X silicone immersion objective), courtesy of Yang Hong Laboratory, Department of Cell Biology, University of Pittsburgh in collaboration with the Center for Biological Imaging. With high-speed scanning, it is possible to minimise photobleaching of the specimen even with long-term imaging.

 

2. Improved specimen viability for longer time-lapse imaging

The AX/AX R features detector units that are twice as sensitive as conventional models*6, with a ~30% reduction in dark current noise*7. With increased sensitivity and reduced noise, lower illumination power can be used for imaging even dim specimens, minimizing photobleaching and phototoxicity. Combined with high-speed resonant scanning which further decreases exposure time, specimen viability is greatly extended, enabling extreme, long-term time-lapse imaging.

 

3. A simplified, AI-driven user experience

Nikon’s NIS-Elements C imaging software allows users to easily customize the layout and experiment workflow to suit their needs. The new Autosignal.ai tool simplifies confocal acquisition by automatically determining the optimal illumination and detection settings while Denoise.ai removes shot noise from confocal images to enable clearer images with shorter exposure times. AI-based tools for post-acquisition image processing such as Segment.ai makes segmentation of complex structures more efficient and reliable.

Graphical programming tools for both acquisition and analysis provide further flexibility for the user. The optional JOBS module enables complex, non-linear experiments with multiple paths and dimensions to be created with ease, including conditional workflows where subsequent acquisition parameters are based on real-time analysis results.

 

For further information please contact us or read more.

 

*1 Galvano scanner
*2 Among point scanners available on the market as of 287 Apirl 2021, according to research conducted by Nikon
*3 The diameter of the observable image in a microscope
*4 ECLIPSE Ti2-E inverted research microscope
*5 ECLIPSE Ni-E motorised upright research microscope
*6 In cases where multi-alkali PMT is selected
*7 Noise generated by the heat of the sensor itself during long exposures

 

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Fixed Wavelength Femtosecond Laser

Coherent Inc introduces Axon 780, the newest wavelegnth in the Axon line of compact, discrete wavelength femtosecond sources.

 

Axon demonstrates market leading performance and features in the same form factor as other Axon lasers, enabling plug-and-play compatibility between wavelengths at the same competitive price point. Like other Axon lasers at 920nm and 1064nm, it is perfect for 2-Photon imaging applications, especially for label free and clinical pathways. It is also ideal for a variety of other applications in nanofabrication, metrology and terahertz spectroscopy.

 

For further information please contact us or read more.

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